BSc, MSc, Licenza (Scuola Normale Superiore), MSc (Oxon), DPhil
Postdoctoral Research Scientist
Investigating the molecular machinery and cellular biology of sleep control
Sleep is vital and universal, yet little is known about its biological functions. A crucial benefit must be provided, in order to balance the enormous risks to which an animal is exposed every time it disconnects from the external world during sleep.
An internal process that generates sleep pressure is tracked by a sleep homeostat, which operates the periodic discharge of this pressure by initiating sleep. Understanding the molecular nature of sleep pressure and its regulation by the homeostat will provide important clues about the physiological variables linked to the essential function of sleep.
Sleep-control neurons innervating the dorsal fan-shaped body (dFBNs) of Drosophila melanogaster's brain constitute the output arm of the fly's sleep homeostat by switching between different electrical states which correlate with the animal’s sleep pressure; they induce or reduce sleep on demand if artificially activated or inhibited.
In his work at Oxford, Raffaele has been combining molecular, transcriptomic, transgenic, imaging and behavioural approaches to investigate the role of dFBNs in sleep control, and further elucidate how sleep is physically encoded in their molecular machinery and circuitry network dynamics.
Given the universal and basic function of sleep, and the striking resemblances between dFBNs and sleep-active cells in the ventrolateral preoptic nucleus of the mammalian hypothalamus, the main findings of these studies could represent general principles conserved across the evolutionary tree.
An in-depth knowledge of the sleep-control processes will inevitably bring insights into the vital functions of sleep.
Raffaele studied at Scuola Normale Superiore and, in parallel, at University of Pisa, in his native Italy, obtaining a BSc in Biotechnologies, an MSc in Molecular and Cell Biology and a Diploma di Licenza in Biology. In Pisa, his research focused on light-controllable engineering of intracellular antibodies, under the supervision of Prof. Antonino Cattaneo.
After a visiting research experience at Harvard Medical School, Raffaele joined the 4-year Wellcome Trust Doctoral Programme in Neuroscience, at Oxford, where he obtained an MSc in Neuroscience, and worked with Prof. Colin Akerman and Prof. Gero Miesenböck for his rotation projects. He then completed a DPhil in Miesenböck lab, at the Centre for Neural Circuits and Behaviour in the Department of Physiology, Anatomy and Genetics, secondarily supervised by Prof. Vladyslav Vyazovskiy. He is currently a Postdoctoral Research Scientist in the Miesenböck group.
In Oxford, Raffaele has been President of the Cortex Club, the neuroscience student association, a unique educational forum dealing with cutting-edge topics and significant, challenging issues in neuroscience with formats ranging from smaller, rather intense debates to large discussion sessions lead by internationally prominent speakers.
During his time in Oxford, Raffaele has received several awards, including the Jesus College Graduate Scholarship, gaining Oxford Scholar status (2019), Feneal UIL Napoli scholarship (2020), the Professional Development Award by the Physiological Society (2023) and a Poster Award by Gordon Research Conferences (2023).
At the same time, he is associate member of the Italian Center for Excellence and Transdisciplinary Studies, and long-term collaborator of Italian National Association of Natural Sciences Teachers, contributing to science communications and public engagement at various levels.