Research groups
Becky Carlyle
Senior Postdoctoral Research Scientist
Research Summary
My research focuses on integration of global measures of RNA (transcriptomics) and protein (mass-spectrometry proteomics) in the post-mortem human brain to understand the molecular changes that leave us susceptible to neurodegeneration and identify biomarkers of disease progression. My current projects focus on:
1. Cerebrospinal fluid biomarkers of Alzheimer's Disease. In collaboration with colleagues at Massachusetts General Hospital and the Yale-NIDA Neuroproteomics Center, I am using data-independent mass-spectrometry to analyse the cerebrospinal fluid of 400 attendees of a neurology clinic, to identify Alzheimer's Disease specific protein signatures from individuals with varied neurological conditions
2. Neuropeptides and resilience to Alzheimer's Disease. In work funded by the Bright Focus Foundation, I am using non-tryptic mass-spectrometry to identify neuropeptides that are highly abundant in individuals who are resilient to amyloid and tau pathology, combined with work in human iPSC-derived neuronal cultures to ascertain if these peptides are actively protective of neuronal function.
3. Spatial proteomics in human post-mortem tissue. I am developing biochemical methods to fractionate human post-mortem brain tissue and mass-spectrometry to identify proteins that may change localisation in disease states.
Biography
Recent publications
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Roadblock: improved annotations do not necessarily translate into new functional insights.
Journal article
Hall NAL. et al, (2021), Genome Biol, 22
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VGF as a biomarker and therapeutic target in neurodegenerative and psychiatric diseases
Journal article
Quinn JP. et al, (2021), Brain Communications
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Synaptic proteins associated with cognitive performance and neuropathology in older humans revealed by multiplexed fractionated proteomics
Journal article
Carlyle BC. et al, (2021), Neurobiology of Aging, 105, 99 - 114
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Evaluation of serological lateral flow assays for severe acute respiratory syndrome coronavirus-2.
Journal article
Trombetta BA. et al, (2021), BMC Infect Dis, 21
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Cerebrovascular Senescence Is Associated With Tau Pathology in Alzheimer’s Disease
Journal article
Bryant AG. et al, (2020), Frontiers in Neurology, 11