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Abnormal oocyte spindle due to the improper function of ooplasm is associated with female infertility of advanced maternal age. A possible way to overcome this problem is to transfer an oocyte germinal vesicle (GV) which contains genetic materials of a patient with a history of poor embryo development to the cytoplast from a donor oocyte. Here we demonstrate that GV transfer is feasible using a rabbit model. When the GVs were transferred to auto- or hetero-cytoplasts of GV stage oocytes, around 80% of the reconstructed oocytes could mature in vitro and 7.1-9.4% of the oocytes developed to blastocyst stage after intracytoplasmic sperm injection (ICSI). Transfer of 93 fertilized eggs reconstructed via GV transfer into six recipients resulted in two live offspring. Results of this experiment indicate that GV transfer can potentially become a new approach in treatment of infertility because of advanced maternal age.

Original publication




Journal article


Mol Reprod Dev

Publication Date





180 - 185


Animals, Cell Nucleus, Cells, Cultured, Cytoplasm, Cytoplasmic Vesicles, Embryo Transfer, Female, Infertility, Female, Meiosis, Oocytes, Pregnancy, Rabbits, Sperm Injections, Intracytoplasmic