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We have examined how herbimycin affects the survival and neuritogenesis of avian sympathetic neurons. Herbimycin promoted sympathetic neuron survival and neuritogenesis. At higher concentrations (> or = 100 ng/ml), herbimycin still enhanced neuron survival but blocked neuritogenesis. Addition of herbimycin (10-30 ng/ml) to neurons cultured in the presence of NGF or retinal conditioned medium altered neuronal morphology, with an increase in the number of neurites. Addition of NGF during hypoxia rescued 52% of the neurons compared to 14% survival in control conditions. Herbimycin alone rescued about 50% of the neurons. In the presence of NGF and 100 ng/ml herbimycin, 81% of the neurons survived hypoxia. Our results show that herbimycin promotes survival of chick sympathetic neurons and potentiates the effects of NGF.

Original publication

DOI

10.1097/00001756-200312190-00022

Type

Journal article

Journal

Neuroreport

Publication Date

19/12/2003

Volume

14

Pages

2397 - 2401

Keywords

Adrenergic Fibers, Animals, Benzoquinones, Cell Death, Cell Hypoxia, Cell Survival, Cells, Cultured, Chick Embryo, Lactams, Macrocyclic, Neurites, Quinones, Rifabutin