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We report on efficient two-photon fluorescence imaging in beam scanning microscopy by exciting UV dyes at the 647-nm line of a continuous wave ArKr mixed gas laser. For a numerical aperture of 1.4 (oil), we used an illumination power of up to 210 mW at the sample. High-resolution images were obtained for DAPI-labelled cell nuclei within 4-60 s. Our method is a simple two-photon alternative to UV confocal imaging with the potential of becoming a very useful feature of laser scanning microscopy.

Type

Journal article

Journal

J Microsc

Publication Date

06/1998

Volume

190

Pages

298 - 304

Keywords

Animals, DNA, Drosophila, Fibroblasts, Indoles, Lasers, Mice, Microscopy, Confocal, Microscopy, Fluorescence, Photons