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We implement wave front sensor-less adaptive optics in a structured illumination microscope. We investigate how the image formation process in this type of microscope is affected by aberrations. It is found that aberrations can be classified into two groups, those that affect imaging of the illumination pattern and those that have no influence on this pattern. We derive a set of aberration modes ideally suited to this application and use these modes as the basis for an efficient aberration correction scheme. Each mode is corrected independently through the sequential optimisation of an image quality metric. Aberration corrected imaging is demonstrated using fixed fluorescent specimens. Images are further improved using differential aberration imaging for reduction of background fluorescence.

Type

Journal article

Journal

Opt Express

Publication Date

23/06/2008

Volume

16

Pages

9290 - 9305

Keywords

Equipment Design, Equipment Failure Analysis, Image Enhancement, Imaging, Three-Dimensional, Lighting, Microscopy, Fluorescence, Optics and Photonics