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Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.


Journal article


Nucleic Acids Res

Publication Date





Animals, Animals, Genetically Modified, Fertilization, Gene Transfer Techniques, Green Fluorescent Proteins, Luminescent Proteins, Male, Ovum, Sperm Injections, Intracytoplasmic, Spermatozoa, Xenopus, Xenopus laevis