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We describe a method of optical refocusing for high numerical aperture (NA) systems that is particularly relevant for confocal and multiphoton microscopy. This method avoids the spherical aberration that is common to other optical refocusing systems. We show that aberration-free images can be obtained over an axial scan range of 70 μm for a 1.4 NA objective lens. As refocusing is implemented remotely from the specimen, this method will enable high axial scan speeds without mechanical interference between the objective lens and the specimen. © 2007 Optical Society of America.

Original publication

DOI

10.1364/OL.32.002007

Type

Journal article

Journal

Optics Letters

Publication Date

15/07/2007

Volume

32

Pages

2007 - 2009