In-Situ measurement of sarcomere length in cardiac myocytes using a two-photon microscope with near-isotropic scan rate
Corbett A., Bub G., Wilson T.
Images are presented showing sarcomere spacing within a living rodent heart. To uniquely identify the sarcomere spacing, two 2D images, offset from each other by 45 degrees, were taken in the region of the myocyte at high frame rate (Figure 1). Though the use of a fast-focussing microscope, accurate measurement of sarcomere length can be achieved regardless of the myocyte orientation. Configuring the system to image orthogonal planes avoids data redundancy and enables imaging at much faster rates than would be possible with a full 3D stack. © 2011 OSA: BODA/NTM/OMP/OTA.