Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

The effects of adenosine on the amylase secretion from rat pancreatic lobules have been studied. Adenosine induces a dose-dependent stimulation on amylase release, which is maximal at a concentration of 10(-4) M. It has been observed a clear inhibition of this secretory action when atropine was added whereas no amylase release was seen in isolated acini after adenosine. The effect of adenosine is completely blocked by the adenosine receptors antagonist theophylline (10(-4) M), but not by dipyridamole (10(-3) M), a drug that inhibits the transport of adenosine into the cell. The increase of amylase secretion induced by adenosine is inhibited by either the relatively selective A1 receptor antagonist PD116,948 (10(-6) M) and by the A2 receptor antagonist PD115,199 (10(-6) M). Significant increases of amylase release are observed after the relatively selective A1 receptor agonist R-PIA (10(-5) M) and after the relatively selective A2 receptor agonist NECA (10(-4) M). Finally, the effect of R-PIA is not modified by coincubation with PD115,199 and the effect of NECA is not affected by coincubation with PD116,948. These results suggest that the action of adenosine is mediated through the release of acetylcholine and probably by the simultaneous occupation of both A1 and A2 adenosine receptors, whereas the intracellular action of adenosine could be discarded.

Type

Journal article

Journal

Life Sci

Publication Date

1995

Volume

57

Pages

PL253 - PL258

Keywords

Adenosine, Adenosine-5'-(N-ethylcarboxamide), Amylases, Animals, Atropine, Dipyridamole, Dose-Response Relationship, Drug, Pancreatin, Purinergic P1 Receptor Antagonists, Rats, Rats, Wistar, Receptors, Purinergic P1, Secretory Rate, Theophylline