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Survival motor neuron (SMN) is the determining factor in spinal muscular atrophy, the most common genetic cause of childhood mortality. We have previously found that SMN regulates stem cell division, proliferation and differentiation in Drosophila. However, it is unknown whether a similar effect exists in vertebrates. Here, we show that SMN is enriched in highly proliferative embryonic stem cells (ESCs) in mice and reduction of SMN impairs the pluripotency of ESCs. Moreover, we find that SMN reduction activates ERK signaling and affects neuronal differentiation in vitro. Teratomas with reduced SMN grow more slowly and show weaker signals of neuronal differentiation than those with a normal level of SMN. Finally, we show that over-expression of SMN is protective for ESCs from retinoic acid-induced differentiation. Taken together, our results suggest that SMN plays a role in the maintenance of pluripotent ESCs and neuronal differentiation in mice.

Original publication

DOI

10.1007/s00429-014-0743-7

Type

Journal article

Journal

Brain Struct Funct

Publication Date

2015

Volume

220

Pages

1539 - 1553

Keywords

Animals, Cell Differentiation, Female, MAP Kinase Signaling System, Male, Mice, Mice, Inbred BALB C, Mouse Embryonic Stem Cells, Neurons, Survival of Motor Neuron 1 Protein, Teratoma