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Herein, we report the use of biodegradable nanoparticles (NPs) containing perfluoro-1,5-crown ether (PFCE), a fluorine-based compound (NP170-PFCE) with the capacity to track cells in vivo by magnetic ressonance imaging (MRI) and efficiently release miRNA. NP170-PFCE complexed with miRNAs accumulate whitin the cell's endolysosomal compartment and interact with higher frequency with argonaute2 (Ago2) and GW182 proteins, which are involved in the biological action of miRNAs, than commercial complexes formed by commercial reagents and miRNA, which in turn accumulate in the cell cytoplasm. The release of miRNA132 (miR132) from the NPs increased 3-fold the survival of endothelial cells (ECs) transplanted in vivo and 3.5-fold the blood perfusion in ischemic limbs relatively to control.

Original publication

DOI

10.1021/nn400171w

Type

Journal article

Journal

ACS Nano

Publication Date

23/04/2013

Volume

7

Pages

3362 - 3372

Keywords

Angiogenesis Inducing Agents, Animals, Cell Survival, Cell Tracking, Cells, Cultured, Endothelial Cells, Hindlimb, Humans, Ischemia, Magnetic Resonance Imaging, Mice, MicroRNAs, Nanocapsules, Transfection