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Stimulated emission depletion (STED) microscopy allows fluorescence far-field imaging with diffraction-unlimited resolution. Unfortunately, extending this technique to three-dimensional (3D) imaging of thick specimens has been inhibited by sample-induced aberrations. Here we present the first implementation of adaptive optics in STED microscopy to allow 3D super-resolution imaging in strongly aberrated imaging conditions, such as those introduced by thick biological tissue.

Type

Journal article

Journal

Opt Express

Publication Date

10/09/2012

Volume

20

Pages

20998 - 21009

Keywords

Animals, Microscopy, Fluorescence, Microspheres, Optical Phenomena, Optics and Photonics, Retina, Zebrafish