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In recent years, new techniques have spurred the discovery of cis-regulatory DNA elements. These stretches of noncoding DNA contain combinations of recognition sites to which transcription factors (TFs) bind, and in doing so, these TFs can activate or repress transcription. These protein-DNA interactions form the core of gene regulatory networks (GRNs) that are responsible for the differential gene expression that allow diversification of cell types, developmental programs, and responses to the environment. The yeast one-hybrid system is a genetic assay to identify direct binding of proteins to DNA elements of interest and is, therefore, instrumental in uncovering these GRNs.

Original publication




Journal article


Methods Mol Biol

Publication Date





335 - 355


DNA, Gene Regulatory Networks, High-Throughput Nucleotide Sequencing, Protein Binding, Regulatory Elements, Transcriptional, Saccharomyces cerevisiae, Transcription Factors, Two-Hybrid System Techniques