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In recent years, new techniques have spurred the discovery of cis-regulatory DNA elements. These stretches of noncoding DNA contain combinations of recognition sites to which transcription factors (TFs) bind, and in doing so, these TFs can activate or repress transcription. These protein-DNA interactions form the core of gene regulatory networks (GRNs) that are responsible for the differential gene expression that allow diversification of cell types, developmental programs, and responses to the environment. The yeast one-hybrid system is a genetic assay to identify direct binding of proteins to DNA elements of interest and is, therefore, instrumental in uncovering these GRNs.

Original publication

DOI

10.1007/978-1-61779-292-2_20

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2012

Volume

786

Pages

335 - 355

Keywords

DNA, Gene Regulatory Networks, High-Throughput Nucleotide Sequencing, Protein Binding, Regulatory Elements, Transcriptional, Saccharomyces cerevisiae, Transcription Factors, Two-Hybrid System Techniques