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Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level.

Original publication

DOI

10.1073/pnas.1111662109

Type

Journal article

Journal

Proc Natl Acad Sci U S A

Publication Date

21/02/2012

Volume

109

Pages

2919 - 2924

Keywords

Animals, Cerebral Cortex, Imaging, Three-Dimensional, Mice, Mice, Inbred C57BL, Microscopy, Fluorescence, Multiphoton, Neurons, Reproducibility of Results