Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

3D organoids have been widely used as tractable in vitro models capable of elucidating aspects of human development and disease. However, the manual and low throughput culture methods coupled with a low reproducibility and geometric heterogeneity restricts the scope and application of organoid research. Combining expertise from stem cell biology and bioengineering offers a promising approach to address some of these limitations. Here, we use melt electrospinning writing to generate tuneable grid scaffolds that can guide the self-organization of pluripotent stem cells into patterned arrays of embryoid bodies. We show that grid geometry is a key determinant of stem cell self-organization, guiding the position and size of emerging lumens via curvature-controlled tissue growth. We report two distinct methods for culturing scaffold-grown embryoid bodies into either interconnected or spatially discrete cerebral organoids. These scaffolds provide a high-throughput method to generate, culture and analyse large numbers of organoids, substantially reducing the time investment and manual labour involved in conventional methods of organoid culture. We anticipate that this methodological development will open up new opportunities for guiding pluripotent stem cell culture, studying lumenogenesis, and generating large numbers of uniform organoids for high throughput screening. This article is protected by copyright. All rights reserved.

Original publication




Journal article


Adv Mater

Publication Date



bioengineering, lumenogenesis, melt electrospinning writing, organoids, scaffolds, stem cells