Peptide-directed spatial organization of biomolecules in dynamic gradient scaffolds.
Chow LW., Armgarth A., St-Pierre J-P., Bertazzo S., Gentilini C., Aurisicchio C., McCullen SD., Steele JAM., Stevens MM.
Specific binding peptides are used to spatially organize biomolecule gradients within an electrospun fiber scaffold. Different biomolecule-binding peptide-polymer conjugates are sequentially co-electrospun with a fiber-forming host polymer to generate opposing gradients of peptide functionalization. The binding peptides specifically and non-covalently guide the spatial arrangement of biomolecules into dynamic gradients within the scaffold, mimicking biological gradients found in native tissues.