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The technique of plasmonic ELISA is utilised here to detect the HIV-1 protein gp120 with the ultralow limit of detection of 8 × 10(-20) M (10(-17) g mL(-1)) in an independent laboratory. It was corroborated that changes in the concentration of hydrogen peroxide as small as 0.05 μM could lead to nanoparticle solutions of completely different tonality.

Original publication

DOI

10.1039/c3nr06167a

Type

Journal article

Journal

Nanoscale

Publication Date

21/08/2014

Volume

6

Pages

9559 - 9562

Keywords

Enzyme-Linked Immunosorbent Assay, HIV Envelope Protein gp120, Hydrogen Peroxide, Limit of Detection, Nanoparticles, Nanotechnology, Poisson Distribution