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Endonuclease G (EndoG) is a mitochondrial enzyme believed to be released during apoptosis to participate in the degradation of nuclear DNA. This paper describes a Drosophila protein, EndoGI, which inhibits EndoG specifically. EndoG and EndoGI associate with subpicomolar affinity, forming a 2:1 complex in which dimeric EndoG is bound by two tandemly repeated homologous domains of monomeric EndoGI. Binding appears to involve the active site of EndoG. EndoGI is present in the cell nucleus at micromolar concentrations. Upon induction of apoptosis, levels of the inhibitor appear to be reduced, and it is relocalized to the cytoplasm. EndoGI, encoded by the predicted open reading frame cg4930, is expressed throughout Drosophila development. Flies homozygous for a hypomorphic EndoGI mutation have a strongly reduced viability, which is modulated by genetic background and diet. We propose that EndoGI protects the cell against low levels of EndoG outside mitochondria.

Original publication

DOI

10.1074/jbc.M808319200

Type

Journal article

Journal

J Biol Chem

Publication Date

27/03/2009

Volume

284

Pages

8337 - 8348

Keywords

Animals, Cell Line, Drosophila Proteins, Drosophila melanogaster, Endodeoxyribonucleases, Enzyme Inhibitors, Mitochondria, Protein Binding, Protein Structure, Tertiary