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Axon outgrowth during development and neurotransmitter release depends on exocytotic mechanisms, although what protein machinery is common to or differentiates these processes remains unclear. Here we show that the neural t-SNARE (target-membrane-associated-soluble N-ethylmaleimide fusion protein attachment protein (SNAP) receptor) SNAP-25 is not required for nerve growth or stimulus-independent neurotransmitter release, but is essential for evoked synaptic transmission at neuromuscular junctions and central synapses. These results demonstrate that the development of neurotransmission requires the recruitment of a specialized SNARE core complex to meet the demands of regulated exocytosis.

Original publication




Journal article


Nat Neurosci

Publication Date





19 - 26


Animals, Brain, Cells, Cultured, Dermis, Diaphragm, Embryo, Mammalian, Embryonic and Fetal Development, Exocytosis, Immunohistochemistry, In Vitro Techniques, Membrane Proteins, Mice, Mice, Knockout, Muscle, Skeletal, Nerve Tissue Proteins, Neuromuscular Junction, Neurons, Patch-Clamp Techniques, SNARE Proteins, Synaptic Transmission, Synaptosomal-Associated Protein 25, Vesicular Transport Proteins