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In the mammalian nervous system, stimulation of G-protein-coupled type I glutamate receptors triggers various forms of neuronal plasticity, including cerebellar long-term depression and hippocampal long-term potentiation. Activation of these receptors in the cerebellum also leads to a slow excitatory postsynaptic current mediated by nonselective TRPC3 cation channels. How TRPC3 channels are opened is unknown, although it is widely thought that channel gating requires phospholipase C activation. Using the patch-clamp technique and immunohistochemistry in rat cerebellar slices, we show that metabotropic glutamate receptors activate TRPC3 channels through the small GTP-binding protein Rho and subsequent phospholipase D stimulation. TRPC3 channel gating is independent of phospholipase C activity. These results reveal a new mechanism for the gating of the ubiquitous TRPC3 channel and identify a key role for phospholipase D in the generation of the slow excitatory postsynaptic current in cerebellar Purkinje cells.

Original publication




Journal article



Publication Date





318 - 325


1-Butanol, Animals, Calcium Signaling, Cell Line, Cerebellum, Excitatory Postsynaptic Potentials, Humans, Immunohistochemistry, In Vitro Techniques, Ion Channel Gating, Patch-Clamp Techniques, Phospholipase D, Purkinje Cells, Rats, Rats, Sprague-Dawley, Receptors, Metabotropic Glutamate, Recombinant Proteins, TRPC Cation Channels, Transfection, Type C Phospholipases, rho GTP-Binding Proteins