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PURPOSE: To assess the potential of a gene reporter system, based on a urea transporter (UTB) and hyperpolarized [(13) C]urea. METHODS: Mice were implanted subcutaneously with either unmodified control cells or otherwise identical cells expressing UTB. After injection of hyperpolarized [(13) C]urea, a spin echo sequence was used to measure urea concentration, T1 , and diffusion in control and UTB-expressing tissue. RESULTS: The apparent diffusion coefficient of hyperpolarized urea was 21% lower in tissue expressing UTB, in comparison with control tissue (P < 0.05, 1-tailed t-test, n = 6 in each group). No difference in water apparent diffusion coefficient or cellularity between these tissues was found, indicating that they were otherwise similar in composition. CONCLUSION: Expression of UTB, by mediating cell uptake of urea, lowers the apparent diffusion coefficient of hyperpolarized (13) C urea in tissue and thus the transporter has the potential to be used as a magnetic resonance-based gene reporter in vivo. Magn Reson Med 73:1401-1406, 2015. © 2014 Wiley Periodicals, Inc.

Original publication

DOI

10.1002/mrm.25254

Type

Journal article

Journal

Magn Reson Med

Publication Date

04/2015

Volume

73

Pages

1401 - 1406

Keywords

13C MRS, gene reporter, hyperpolarization, urea transporter, Animals, Carbon Isotopes, HEK293 Cells, Humans, Magnetic Resonance Spectroscopy, Membrane Transport Proteins, Mice, Mice, SCID, Reproducibility of Results, Sensitivity and Specificity, Tissue Distribution, Transgenes, Urea