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The intermediate filament-like protein syncoilin is a member of the dystrophin protein complex, and links the complex to the cytoskeleton through binding alpha-dystrobrevin and desmin in muscle. Here, we identify further sites of syncoilin location in normal muscle: at the perinuclear space, myotendinous junction, and enrichment in the sarcolemma and sarcoplasm of oxidative muscle fibers in mice. To understand the importance of the dystrophin protein complex-syncoilin-cytoskeletal link and its implication to disease, we analyzed syncoilin in mice null for alpha-dystrobrevin (adbn-/-) and desmin (des-/-). Syncoilin was upregulated in dystrophic muscles of adbn-/- mice, without alteration in its subcellular location. In des-/- mice, syncoilin was severely reduced in skeletal muscle; lost from sarcomeric Z-lines and neuromuscular junctions, and redistributed from the sub-sarcolemmal cytoskeleton to the cytoplasm. The data show that absence of alpha-dystrobrevin or desmin leads to dynamic changes in syncoilin that may compensate for, or participate in, different muscle myopathies.

Original publication

DOI

10.1016/j.nmd.2007.06.004

Type

Journal article

Journal

Neuromuscul Disord

Publication Date

12/2007

Volume

17

Pages

970 - 979

Keywords

Animals, Cytoplasm, Desmin, Dystrophin-Associated Proteins, Intermediate Filament Proteins, Mice, Mice, Inbred C57BL, Mice, Knockout, Microscopy, Electron, Transmission, Muscle Fibers, Skeletal, Muscle Proteins, Muscle, Striated, Muscular Diseases, Neuromuscular Junction, Sarcolemma, Sarcomeres, Up-Regulation