Search results
Found 10671 matches for
-
beta-Exodomain Role in Na/K-ATPase alpha-beta Subunit Interactions
11 September 2018
-
Mutational signature distribution varies with DNA replication timing and strand asymmetry.
1 October 2018
BACKGROUND: DNA replication plays an important role in mutagenesis, yet little is known about how it interacts with other mutagenic processes. Here, we use somatic mutation signatures-each representing a mutagenic process-derived from 3056 patients spanning 19 cancer types to quantify the strand asymmetry of mutational signatures around replication origins and between early and late replicating regions. RESULTS: We observe that most of the detected mutational signatures are significantly correlated with the timing or direction of DNA replication. The properties of these associations are distinct for different signatures and shed new light on several mutagenic processes. For example, our results suggest that oxidative damage to the nucleotide pool substantially contributes to the mutational landscape of esophageal adenocarcinoma. CONCLUSIONS: Together, our results indicate an interaction between DNA replication, the associated damage repair, and most mutagenic processes.
-
Monitoring real-time hormone release kinetics via high-content 3-D imaging of compensatory endocytosis.
1 October 2018
High-content real-time imaging of hormone secretion in tissues or cell populations is a challenging task, which is unlikely to be resolved directly, despite immense translational value. We approach this problem indirectly, using compensatory endocytosis, a process that closely follows exocytosis in the cell, as a surrogate read-out for secretion. The tissue is immobilized in an open-air perifusion chamber and imaged using a two-photon microscope. A fluorescent polar tracer, perifused through the experimental circuit, gets trapped into the cells via endocytosis, and is quantified using a feature-detection algorithm. The signal of the tracer that accumulates into the endocytotic system reliably reflects stimulated exocytosis, which is demonstrated via co-imaging of the latter using existing reporters. A high signal-to-noise ratio and compatibility with multisensor imaging affords the real-time quantification of the secretion at the tissue/population level, whereas the cumulative nature of the signal allows imprinting of the "secretory history" within each cell. The technology works for several cell types, reflects disease progression and can be used for human tissue.
-
Light modulation ameliorates expression of circadian genes and disease progression in spinal muscular atrophy mice.
1 August 2018
Physiology and behaviour are critically dependent on circadian regulation via a core set of clock genes, dysregulation of which leads to metabolic and sleep disturbances. Metabolic and sleep perturbations occur in spinal muscular atrophy (SMA), a neuromuscular disorder caused by loss of the survival motor neuron (SMN) protein and characterised by motor neuron loss and muscle atrophy. We therefore investigated the expression of circadian rhythm genes in various metabolic tissues and spinal cord of the Taiwanese Smn-/-;SMN2 SMA animal model. We demonstrate a dysregulated expression of the core clock genes (clock, ARNTL/Bmal1, Cry1/2, Per1/2) and clock output genes (Nr1d1 and Dbp) in SMA tissues during disease progression. We also uncover an age- and tissue-dependent diurnal expression of the Smn gene. Importantly, we observe molecular and phenotypic corrections in SMA mice following direct light modulation. Our study identifies for a key relationship between a SMA pathology and peripheral core clock gene dysregulation, highlights the influence of SMN on peripheral circadian regulation and metabolism and has significant implications for the development of peripheral therapeutic approaches and clinical care management of SMA patients.
-
Group photos
8 September 2014
-
Celebrations
8 September 2014
-
Publications
30 April 2015
-
Useful links
30 April 2015
-
Alumni
30 April 2015
-
Lab pictures
30 April 2015
-
OXfCRISPR - Oxford fly CRISPR resources
30 April 2015
-
News
30 April 2015
-
CRISPR Community
30 April 2015
-
Ralph W. Gerard Prize in Neuroscience recognizes outstanding contributions of Colin Blakemore
13 October 2012
Blakemore receives $25,000 award for contributions to neuroscience
-
Switching on a light in the brain
27 November 2012
A new technique known as optogenetics is lighting up the field of neuroscience. The idea involves genetically engineering neurons to respond to light, and then using powerful lasers to stimulate and control their expression.
-
Unlocking the Brain
12 March 2014
As part of Brain Awareness Week 2014, the auditory neuroscience group in DPAG is putting on a series of interactive activities that include visualizing your own speech sounds and demonstrating how are ears and brains process these sounds.
-
Neural pathways in complex mating rituals identified in fruit flies
16 November 2016
Scientists have identified the neural pathway in male fruit flies that allows them to perform their complex mating ritual, paving the way for deeper studies into sexual behavior and how it can be modified by social experience.
-
Antonio Miranda Shortlisted for National Image Competition
15 June 2016
Cardiac Theme Outreach Research
An image of a whole embryonic mouse heart at 13 days after fertilisation has been shortlisted in a competition run by the British Heart Foundation (BHF).
-
Andrew Parker to Give Physiological Society's GL Brown Prize Lecture
18 May 2017
Our congratulations to Professor Andrew Parker who has been asked to deliver the Physiological Society's GL Brown prize lecture.
-
Lizzie Burns & DPAG recognised in OxTALENT 2015
26 June 2015
Lizzie Burns is the joint winner of the "Outreach and Public Engagement: TES Connect" category for her work in collaboration with Zoltan Molnar and Damion Young entitled ‘CSlide’: a Nobel Prize-Winner Inspires Science, Art and Creativity.
-
Research
2 August 2018
-
About us
30 August 2018
-
Study with us
24 January 2018
-
Molnar Group Images
5 September 2016
-
Publication Front Covers
18 November 2015
-
Media
10 July 2015
-
Collaborations and Funding
26 September 2014
-
The Seeing Garden Oxford
8 April 2015
-
Lab News
22 October 2015
-
Photos
10 July 2015
-
El Andaloussi Group
16 September 2013
Development of delivery vectors for therapeutic nucleic acids and proteins
-
Evans Group
10 July 2016
Role of triacylglycerol-rich lipoproteins in substrate supply and metabolic signalling
-
Glitsch Group
2 April 2014
Proton dependence of intracellular calcium signalling in the cerebellum in health and disease - role of extracellular pH sensing receptors and ion channels.
-
Goberdhan Group
10 July 2016
Growth Regulation and Cancer: mTORC1, Exosomes and Cellular Amino Acid Sensing
-
MRC FGU Public engagement
Find out more about the Becker Group and the Functional Genomics Unit's public engagement here.