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Legend: Lack of tangential migration of glutamatergic (left) but not GABAergic (right) neurons during chick brain development. The left diagram demonstrates that no tangentially migrating glutamatergic neurons leave from the labelled segment of the neuroepithelium (cortical haem) in the embryonic chick brain, which normally produce such neurons in mammals. The right panels demonstrate that GABAergic interneurons (green) are the only population that arrives tangentially to the dorsal area of the forebrain (red) from external sources. Interneurons were labeled at their site of origin in the subpallium with a plasmid DNA encoding the green fluorescente protein. The dorsal area of the forebrain is labelled in red by immunohistochemistry against the transcription factor Satb2.
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