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We provide evidence that ablation or inhibition of, uncoupling protein 1 increases the rate of reactive oxygen containing species production by mitochondria from brown adipose tissue, no matter what electron transport chain substrate is used (succinate, glycerol-3-phosphate or pyruvate/malate). Consistent with these data are our observations that (a) the mitochondrial membrane potential is maximal when uncoupling protein 1 is ablated or inhibited and (b) oxygen consumption rates in mitochondria from uncoupling protein 1 knock-out mice, are significantly lower than those from wild-type mice, but equivalent to those from wild-type mice in the presence of GDP. In summary, we show that uncoupling protein 1 can affect reactive oxygen containing species production by isolated mitochondria from brown adipose tissue.

Original publication




Journal article


Biochim Biophys Acta

Publication Date





1470 - 1476


Adipose Tissue, Brown, Animals, Female, Ion Channels, Male, Mice, Mice, Inbred C57BL, Mitochondria, Mitochondrial Proteins, Oxygen Consumption, Reactive Oxygen Species