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During NMR experiments on isolated, perfused rat heart, we observed a 57% change in probe sensitivity when the perfusion buffer surrounding the heart was replaced by either a low-ionic-strength mannitol solution or air and the heart was made ischemic. Such changes in sensitivity, should they go unrecognized, would result in substantial quantification errors.

Type

Journal article

Journal

Magn Reson Med

Publication Date

10/1992

Volume

27

Pages

343 - 348

Keywords

Air, Animals, Cardioplegic Solutions, Glucose, Magnetic Resonance Spectroscopy, Male, Mannitol, Myocardial Ischemia, Myocardium, Organophosphonates, Osmolar Concentration, Perfusion, Rats, Rats, Sprague-Dawley, Tromethamine