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Monoclonal antibodies (mAb) have great potential value for in vivo diagnosis and therapy in humans. Their antigenic nature is however responsible for severe side effects and successful applications in the clinic have prove to be more limited than was originally hoped. This review summarize both cell biology and molecular biology approaches developed in order to overcome these limitations. Improving methodologies to immortalize cell lines producing human mAbs are reported with a particular attention to the techniques aimed at rescuing B cells expressing high-affinity human antibodies. A major part of this review is devoted to the protein engineering work. Genetic manipulation of mouse monoclonals to produce humanized antibodies and preparation of bacteriophage libraries displaying Ig repertoires are examined. The possibility to extend these approaches to the production of in vitro repertoires and to obviate the in vivo immunization step is also discussed. © 1993 Springer-Verlag.

Original publication

DOI

10.1007/BF02592308

Type

Journal article

Journal

International Journal of Clinical and Laboratory Research

Publication Date

01/12/1993

Volume

23

Pages

192 - 198