Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we will assume that you are happy to receive all cookies and you will not see this message again. Click 'Find out more' for information on how to change your cookie settings.

Small interfering RNA (siRNA) is a powerful tool for modulating gene expression by RNA interference (RNAi). Duplex RNA oligonucleotides induce cleavage of homologous target transcripts, thereby enabling posttranscriptional silencing of potentially any gene. As such, siRNAs may have utility as novel pharmaceuticals for a wide range of diseases. However, a lack of "drug-likeness," physiological barriers, and potential toxicities have meant that systemic delivery of SiRNAs in vivo remains a major challenge. Here we discuss various strategies that have been employed to solve the problem of SiRNA delivery. These include chemical modification of the SiRNA, direct conjugation to bioactive moieties, and nanoparticle formulations.

Original publication

DOI

10.1007/978-1-4939-3112-5_23

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2016

Volume

1364

Pages

291 - 310

Keywords

Cell-penetrating peptides, Lipid nanoparticles, Lipidoids, SiRNA conjugates, SiRNA delivery, Animals, Drug Carriers, Hydrophobic and Hydrophilic Interactions, RNA, Small Interfering