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The antiproliferative action of the guanine-specific ribonuclease secreted by Bacillus intermedius (binase) was studied in different chicken and mouse cell lines. The proliferation rate of chicken embryo fibroblasts, either normal or Rous sarcoma virus-transformed, was significantly reduced by binase treatment. Among mouse fibroblasts, v-ras-transformed NIH3T3 cells were sensitive to binase, whereas the growth of non-transformed, v-src-transformed or v-fms-transformed NIH3T3 cells was not affected. A 48 h treatment with binase inhibited the Ca2+-dependent K+ current of v-ras-transformed NIH3T3 cells but had no effect on this membrane current in non-transformed and in v-src- or v-fms-transformed NIH3T3 cells. Our results suggest that mammalian cells expressing the ras-oncogene are a potential target for the antiproliferative action of binase.

Type

Journal article

Journal

Toxicology

Publication Date

02/01/2001

Volume

156

Pages

101 - 107

Keywords

3T3 Cells, Animals, Bacillus, Calcium, Cell Division, Cell Survival, Cell Transformation, Viral, Cells, Cultured, Chick Embryo, Dose-Response Relationship, Drug, Membrane Potentials, Mice, Patch-Clamp Techniques, Potassium Channels, Ribonucleases