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We introduce an imaging modality that, by offsetting pixel-exposure times during capture of a single image frame, embeds temporal information in each frame. This allows simultaneous acquisition of full-resolution images at native detector frame rates and high-speed image sequences at reduced resolution, without increasing bandwidth requirements. We demonstrate this method using macroscopic and microscopic examples, including imaging calcium transients in heart cells at 250 Hz using a 10-Hz megapixel camera.

Original publication

DOI

10.1038/nmeth.1429

Type

Journal article

Journal

Nat Methods

Publication Date

03/2010

Volume

7

Pages

209 - 211

Keywords

Animals, Calcium, Diagnostic Imaging, Image Interpretation, Computer-Assisted, Microscopy, Fluorescence, Rats, Signal Processing, Computer-Assisted