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To investigate the effects of age and disease on endogenous cardiac progenitor cells, we obtained right atrial and left ventricular epicardial biopsies from patients (n = 22) with chronic ischaemic heart disease and measured doubling time and surface marker expression in explant- and cardiosphere-derived cells (EDCs, CDCs). EDCs could be expanded from all atrial biopsy samples, but sufficient cells for cardiosphere culture were obtained from only 8 of 22 ventricular biopsies. EDCs from both atrium and ventricle contained a higher proportion of c-kit+ cells than CDCs, which contained few such cells. There was wide variation in expression of CD90 (atrial CDCs 5-92 % CD90+; ventricular CDCs 11-89 % CD90+), with atrial CDCs cultured from diabetic patients (n = 4) containing 1.6-fold more CD90+ cells than those from non-diabetic patients (n = 18). No effect of age or other co-morbidities was detected. Thus, CDCs from atrial biopsies may vary in their therapeutic potential.

Original publication

DOI

10.1007/s12265-012-9389-0

Type

Journal article

Journal

J Cardiovasc Transl Res

Publication Date

10/2012

Volume

5

Pages

678 - 687

Keywords

Adult, Aged, Aged, 80 and over, Antigens, Thy-1, Biomarkers, Biopsy, Cell Culture Techniques, Cell Differentiation, Cell Proliferation, Cell Separation, Chronic Disease, Diabetes Mellitus, Female, Flow Cytometry, Heart Atria, Heart Ventricles, Humans, Hypercholesterolemia, Hypertension, Male, Middle Aged, Myocardial Ischemia, Pericardium, Proto-Oncogene Proteins c-kit, Severity of Illness Index, Smoking, Spheroids, Cellular, Stem Cells