Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we will assume that you are happy to receive all cookies and you will not see this message again. Click 'Find out more' for information on how to change your cookie settings.

Optical imaging of physiological events in real time can yield insights into biological function that would be difficult to obtain by other experimental means. However, the detection of all-or-none events, such as action potentials or vesicle fusion events, in noisy single-trial data often requires a careful balance of tradeoffs. The analysis of such experiments, as well as the design of optical reporters and instrumentation for them, is aided by an understanding of the principles of signal detection. This review illustrates these principles, using as an example action potential recording with optical voltage reporters.

Original publication

DOI

10.1152/physiol.00036.2006

Type

Journal article

Journal

Physiology (Bethesda)

Publication Date

02/2007

Volume

22

Pages

47 - 55

Keywords

Action Potentials, Animals, Microscopy, Confocal, Models, Theoretical, Neurons, Optics and Photonics, Photons