Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we will assume that you are happy to receive all cookies and you will not see this message again. Click 'Find out more' for information on how to change your cookie settings.

Impaired cardiac muscle growth and aberrant myocyte arrangement underlie congenital heart disease and cardiomyopathy. We show that cardiac-specific inactivation of the murine homeobox transcription factor Prox1 results in the disruption of expression and localisation of sarcomeric proteins, gross myofibril disarray and growth-retarded hearts. Furthermore, we demonstrate that Prox1 is required for direct transcriptional regulation of the genes encoding the structural proteins alpha-actinin, N-RAP and zyxin, which collectively function to maintain an actin-alpha-actinin interaction as the fundamental association of the sarcomere. Aspects of abnormal heart development and the manifestation of a subset of muscular-based disease have previously been attributed to mutations in key structural proteins. Our study reveals an essential requirement for direct transcriptional regulation of sarcomere integrity, in the context of enabling foetal cardiomyocyte hypertrophy, maintenance of contractile function and progression towards inherited or acquired myopathic disease.

Original publication

DOI

10.1242/dev.030007

Type

Journal article

Journal

Development

Publication Date

02/2009

Volume

136

Pages

495 - 505

Keywords

Actinin, Animals, Embryo, Mammalian, Gene Expression Regulation, Developmental, Heart, Heart Defects, Congenital, Homeodomain Proteins, Metalloproteins, Mice, Mice, Transgenic, Muscle Proteins, Myocardial Contraction, Myocardium, Sarcomeres, Tumor Suppressor Proteins, Zyxin