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We report on the use of high resolution Raman spectroscopy mapping combined with a micro-engineered stem cell platform. This technique obtains quantitative information about the concentration of individual intracellular molecules such as proteins, lipids, and other metabolites, while tightly controlling cell shape and adhesion. This new quantitative analysis will prove highly relevant for in vitro drug screening applications and regenerative medicine.

Original publication

DOI

10.1039/c4an02346c

Type

Journal article

Journal

Analyst

Publication Date

21/03/2015

Volume

140

Pages

1798 - 1803

Keywords

Cell Line, Cell Shape, Humans, Lipids, Mesenchymal Stem Cells, Proteins, Spectrum Analysis, Raman