Analysis of epicardial genes in embryonic mouse hearts with ﬂow cytometry
REDPATH A., Lupu I-E., SMART N.
Genetic markers used to deﬁne discrete cell populations are seldom expressed exclusively in the population of interest and are, thus, unsuitable when evaluated individually, especially in the absence of spatial and morphological information. Here, we present ﬂuorescence in situ hybridization for ﬂow cytometry to allow simultaneous analysis of multiple marker genes at the single whole-cell level, exempliﬁed by application to the embryonic epicardium. The protocol facilitates multiplexed quantiﬁcation of gene and protein expression and temporal changes across speciﬁc cell populations.