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We have investigated the humoral immune response to E1-deleted adenovirus vectors encoding the lacZ gene introduced into the brains of mice. Injection of these non-replicating vectors into the brain induced systemic antibody production to adenovirus vectors in dose dependent manner. Apparent antibody production to beta-galactosidase, the product of the lacZ gene, was detected later than anti-adenovirus antibody. Neutralizing antibody was not detected. This study demonstrates that E1-deleted adenovirus vectors injected into the brain trigger humoral immune responses to the adenovirus and its gene products, but they are not sufficient to block the infection of cells by adenovirus upon repeat injection.

Type

Journal article

Journal

J Neuroimmunol

Publication Date

01/02/2000

Volume

103

Pages

8 - 15

Keywords

Adenoviridae, Animals, Antibodies, Viral, Brain, Genetic Therapy, Genetic Vectors, Immunohistochemistry, Male, Mice, Mice, Inbred C3H, beta-Galactosidase