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One of the long-term goals of mutagenesis programs in the mouse has been to generate mutant lines to facilitate the functional study of every mammalian gene. With a combination of complementary genetic approaches and advances in technology, this aim is slowly becoming a reality. One of the most important features of this strategy is the ability to identify and compare a number of mutations in the same gene, an allelic series. With the advent of gene-driven screening of mutant archives, the search for a specific series of interest is now a practical option. This review focuses on the analysis of multiple mutations from chemical mutagenesis projects in a wide variety of genes and the valuable functional information that has been obtained from these studies. Although gene knockouts and transgenics will continue to be an important resource to ascertain gene function, with a significant proportion of human diseases caused by point mutations, identifying an allelic series is becoming an equally efficient route to generating clinically relevant and functionally important mouse models.

Original publication

DOI

10.1007/s00335-007-9014-8

Type

Journal article

Journal

Mamm Genome

Publication Date

07/2007

Volume

18

Pages

412 - 424

Keywords

Animals, Genetic Diseases, Inborn, Genotype, Humans, Mice, Mice, Transgenic, Mutant Proteins, Phenotype