Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we will assume that you are happy to receive all cookies and you will not see this message again. Click 'Find out more' for information on how to change your cookie settings.

Intracellular pH (pH(i)) is an important modulator of cardiac function. Because it is readily influenced by metabolic processes, pH(i) is controlled physiologically. Classical models of intracellular pH regulation comprise acid/base transport proteins expressed in the sarcolemma, acting in concert with intracellular buffers. These two processes are coupled via a diffusive movement of protons. Because intracellular H(+) buffering is high, H(i)(+)-diffusion occurs through a passive shuttling on intrinsic mobile buffers such as acetylated carnosine, anserine and homocarnosine: low molecular weight imidazole compounds. This mechanism is assisted by carbonic buffer, a system regulated biochemically by the enzyme carbonic anhydrase. H(i)(+)-mobility via the buffer shuttles is low, and this can result in significant pH(i) non-uniformity under conditions of high proton flux across the sarcolemma or within the cell. Spatial regulation of pH(i) is complemented by passive H(+) permeation between cells through gap junctions. This permeation is also mediated via protonated buffers. The control of pH(i) is therefore dependent on carrier molecules that spatially shuttle protons within and between cells. In this review, we consider the physiological regulation of H(i)(+)-mobility and permeation, and its relevance to pH(i)-control in normal and pathophysiological states such as myocardial ischaemia, a clinical condition associated with severe intracellular acidosis.

Original publication

DOI

10.1016/j.pbiomolbio.2005.06.004

Type

Journal article

Journal

Prog Biophys Mol Biol

Publication Date

01/2006

Volume

90

Pages

207 - 224

Keywords

Animals, Carbonic Anhydrases, Cell Communication, Cell Compartmentation, Gap Junctions, Guinea Pigs, Hydrogen-Ion Concentration, Models, Biological, Myocardium, Myocytes, Cardiac, Protons, Rats, Sarcolemma