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Basic and clinical studies have shown that bone marrow cell therapy can improve cardiac function following infarction. In experimental animals, reported stem cell-mediated changes range from no measurable improvement to the complete restoration of function. In the clinic, however, the average improvement in left ventricular ejection fraction is around 2% to 3%. A possible explanation for the discrepancy between basic and clinical results is that few basic studies have used the magnetic resonance (MR) imaging (MRI) methods that were used in clinical trials for measuring cardiac function. Consequently, we employed cine-MR to determine the effect of bone marrow stromal cells (BMSCs) on cardiac function in rats. Cultured rat BMSCs were characterized using flow cytometry and labeled with iron oxide particles and a fluorescent marker to allow in vivo cell tracking and ex vivo cell identification, respectively. Neither label affected in vitro cell proliferation or differentiation. Rat hearts were infarcted, and BMSCs or control media were injected into the infarct periphery (n = 34) or infused systemically (n = 30). MRI was used to measure cardiac morphology and function and to determine cell distribution for 10 wk after infarction and cell therapy. In vivo MRI, histology, and cell reisolation confirmed successful BMSC delivery and retention within the myocardium throughout the experiment. However, no significant improvement in any measure of cardiac function was observed at any time. We conclude that cultured BMSCs are not the optimal cell population to treat the infarcted heart.

Original publication

DOI

10.1152/ajpheart.00094.2008

Type

Journal article

Journal

Am J Physiol Heart Circ Physiol

Publication Date

08/2008

Volume

295

Pages

H533 - H542

Keywords

Animals, Bone Marrow Cells, Bone Marrow Transplantation, Carbocyanines, Cell Differentiation, Cell Movement, Cell Proliferation, Cell Survival, Cells, Cultured, Disease Models, Animal, Ferric Compounds, Flow Cytometry, Fluorescent Dyes, Green Fluorescent Proteins, Immunohistochemistry, Magnetic Resonance Imaging, Cine, Male, Myocardial Contraction, Myocardial Infarction, Myocardium, Rats, Rats, Wistar, Staining and Labeling, Stroke Volume, Stromal Cells, Time Factors, Ventricular Function, Left