Dissection of embryonic mouse kidney, culture in vitro, and imaging of the developing organ.
Costantini F., Watanabe T., Lu B., Chi X., Srinivas S.
In this article, we outline procedures for the dissection of intact kidneys and the isolation of the ureteric bud (UB) and the metanephric mesenchyme (MM) from mouse embryos. The apparatus required for the culture of these tissues in vitro is described in detail as well as the equipment necessary for performing time-lapse imaging studies of the developing kidney.