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FRET imaging

FRAP in colorectal cancer cell lines

Stefania Monterisi


Postdoctoral Research Scientist

Research Interests

I am a cellular and molecular biologist with expertise in intracellular signalling. My current field of research is colorectal cancer and my project is focused on investigating the role of proton signalling in cancer development, by measuring intracellular and extracellular pH in a panel of cancer cell lines; the heterogeneous expression of genes involved in pH regulation and how cells communicate by being coupled via gap junctions. 

In my previous research I looked at the compartmentalisation of cAMP/PKA signalling in different cell models. In cardiac myocytes I investigated the role of phosphodiesterases (PDEs), the enzymes that degrade both cAMP and cGMP, in cardiac hypertrophy and heart failure. In particular I studied the effect of PDE2 on mitochondria morphology and cell survival.

In cystic fibrosis airway cells my interest was directed to the activation pathway of CFTR and its interactome.

My technical skills are: FRET and FRAP imaging, biochemistry- WB, molecular biology techniques -PCR, genotyping, cell death/cell viability assays, confocal imaging, histology, FACS, xenografts, mini-pump surgery, and the generation of genetically modified mice as tool to understand the effect of protein knock out in vivo.


I earned my Master degree in Biological Sciences from the University of Bari, Italy. I obtained my PhD in Molecular and Cellular Biotechnologies from the same University, after spending part of my doctoral training at the Venetian Institute of Molecular Medicine in Padova, Italy, working on the alteration of signal transduction in Cystic Fibrosis.

I then received a fellowship to work at Glasgow University, UK, to refine my skills in FRET imaging.

I moved to Oxford in 2012 for a post-doctoral position, joining the group of Prof. Manuela Zaccolo to work on cyclic nucleotides signalling in heart disease. 

From July 2017 I joined the group of Prof. Pawel Swietach to work on pH signalling in cancer, in collaboration with Prof. Sir Walter Bodmer at the WIMM.

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