Fluorescence spectroscopy and electrophysiology of the KATP channel
The ATP-sensitive potassium (KATP) channel couples the metabolic state of a cell to its electrical activity. The regulation of the channel by the ratio of intracellular adenine nucleotides (ATP:ADP) is critical for insulin secretion and blood glucose homeostasis.
To study the binding of nucleotides to KATP, I use a combination of fluorescence spectroscopy and electrophysiology with channels incorporating a non-canonical, fluorescent amino acid.
I hope to further our understanding of the biophysical properties of the channel, and how mutations which cause diabetes or hyperinsulinemia can disrupt its regulation.