Fluorescence spectroscopy and electrophysiology of the KATP channel
The ATP-sensitive potassium (KATP) channel couples the metabolic state of a cell to its electrical activity. The regulation of the channel by the ratio of intracellular adenine nucleotides (ATP:ADP) is critical for insulin secretion and blood glucose homeostasis.
To study the binding of nucleotides to KATP, I use a combination of fluorescence spectroscopy and electrophysiology with channels incorporating a non-canonical, fluorescent amino acid.
I hope to further our understanding of the biophysical properties of the channel, and how mutations which cause diabetes or hyperinsulinemia can disrupt its regulation.
Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time.
Usher SG. et al, (2021), J Vis Exp
New insights into KATP channel gene mutations and neonatal diabetes mellitus.
Pipatpolkai T. et al, (2020), Nat Rev Endocrinol, 16, 378 - 393
Activation mechanism of ATP-sensitive K+ channels explored with real-time nucleotide binding.
Puljung M. et al, (2019), Elife, 8
FTO influences adipogenesis by regulating mitotic clonal expansion.
Merkestein M. et al, (2015), Nat Commun, 6