- Two-photon microscopy
- Rodent intracranial microsurgery
- Head-fixed rodent in vivo imaging
- ImageJ analysis and scripting
Postdoctoral Research Scientist
Rob obtained his Master’s in Science in Biochemistry from the University of Birmingham, Birmingham, UK. There he developed his skills with multiple modalities of fluorescence microscopy including TIRF and confocal, to study the mechanisms of metastatic breast cancer cell migration with Dr Joshua Rappoport.
Subsequently, Rob obtained his PhD in cellular neurobiology as part of the Wellcome Trust Dynamic Cell Biology programme at the University of Bristol, Bristol, UK. There, under the supervision of Dr Michael Ashby, he investigated the role of mitochondrial positioning in the structural plasticity of presynaptic terminals in adult mouse neocortex using in vivo two-photon microscopy.
As part of his postdoctoral research, he is based in the Packer group working on a project to determine how neurons are functionally connected within and across neocortical brain areas in the mouse. This involves cutting-edge all-optical approaches, combining two-photon calcium imaging and two-photon optogenetics in vivo to target individual neurons for photostimulation and record network activity.
The aim of his work is to determine the way in which neurons must be stimulated, both individually and in groups, to elicit downstream responses. By answering these questions we can further understand how neurons integrate information across layers and areas to most efficiently transmit that information.
Presynaptic boutons that contain mitochondria are more stable
LEES R. et al, (2019), bioRxiv
Correlative two-photon and serial block face scanning electron microscopy in neuronal tissue using 3D near-infrared branding maps
Targeted siRNA Screens Identify ER-to-Mitochondrial Calcium Exchange in Autophagy and Mitophagy Responses in RPE1 Cells
MacVicar T. et al, International Journal of Molecular Sciences, 16, 13356 - 13380