My work involves the use of the translating ribosome affinity purification (TRAP) technique to isolate ribosome-bound messenger RNA from specific cell types of interest. The Wade-Martins lab have developed a transgenic Parkinson’s disease mouse model that recapitulates the age-dependent loss of dopaminergic neurons in the midbrain. Using TRAP, I obtain transcriptomic data from dopamine neurons in young and aged, healthy and Parkinsonian mice. I have optimised the technique to obtain RNA from dopaminergic axons projecting to the dorsal and ventral striatum. This allows me to investigate specific cell compartment differences in translation as vulnerable neurons transition from a healthy to diseased and young to aged state.
I qualified as a veterinarian in 2017 from the University of Bristol, where I also completed obtained my bachelor’s degree in Neuroscience. There, I worked under the supervision of Dr Zuner Bortolotto, using electrophysiology to investigate the therapeutic activity of kainate receptor antagonists on epileptiform activity.