Postdoctoral Research Scientist
I am a postdoctoral researcher in Professor Matthew Wood’s lab working on translational research for neuromuscular disorders, more specifically on Myotonic Dystrophy type 1 (DM1).
I completed my PhD on proteomic analysis of animal models of neuromuscluar disorders, including Duchenne muscular dystrophy (DMD) and amyotrophic lateral sclerosis (ALS), in Professor Kay Ohlendieck’s Muscle Biology lab at Maynooth University, Ireland (2011-2015). My first postdoctoral research project centred on defining the roles and mechanisms of action of Pellino proteins in immunity and inflammatory diseases in Professor Paul Moynagh’s Molecular Immunology lab at Maynooth University, Ireland (2015-2017).
My current research focuses on the pre-clinical development of a gene therapy for DM1 using cell penetrating peptides conjugated to phosphorodiamidate morpholino oligonucleotides (PMO). Our aim is to develop a therapy that provides an efficient and safe delivery of next generation antisense oligonucleotides targeting the toxic CTG repeat expansion sequences specific for the treatment of DM1.
My research interests also include exploring the DM1 proteome and identifying novel pathological pathways that contribute to the pathophysiology of DM1 and to investigate novel therapeutic strategies in order to correct the defects.
Comparative Testis Tissue Proteomics Using 2-Dye Versus 3-Dye DIGE Analysis.
Holland A., (2018), 1664, 185 - 202
Pathoproteomic profiling of the skeletal muscle matrisome in dystrophinopathy associated myofibrosis.
Holland A. et al, (2016), Proteomics, 16, 345 - 366
Label-free mass spectrometric analysis of the mdx-4cv diaphragm identifies the matricellular protein periostin as a potential factor involved in dystrophinopathy-related fibrosis
Holland A. et al, (2015), PROTEOMICS, 15, 2318 - 2331
Comparative Label-Free Mass Spectrometric Analysis of Mildly versus Severely Affected mdx Mouse Skeletal Muscles Identifies Annexin, Lamin, and Vimentin as Universal Dystrophic Markers
Holland A. et al, (2015), Molecules, 20, 11317 - 11344
Comparative profiling of the sperm proteome
Holland A. and Ohlendieck K., (2015), PROTEOMICS, 15, 632 - 648