Heart muscle disease: spatio-temporal dynamics of cyclic nucleotides in cardiac pathology
Supervisor: Professor Manuela Zaccolo
The project will test the hypothesis that alteration in the local control of cyclic nucleotide signalling in cardiac myocytes leads to heart disease and will explore the possibility to use local manipulation of cyclic nucleotide signals as a novel therapeutic approach. The study will involve state-of-the-art imaging techniques including real-time imaging of cyclic nucleotides by Fluorescence Resonance Energy Transfer (FRET) and the development of targeted FRET reporters for monitoring of cyclic nucleotide signalling dynamics within distinct subcellular. The video-imaging recordings and a combination of biochemical and genetic approaches will be applied to both in vitro and in vivo models of cardiac disease.
Funding: Applicants are expected to compete for local studentships
Contact details: Dr Manuela Zaccolo
Applications by: 4th January 2013 https://apply.embark.com/grad/Oxford/16/
The focus of the laboratory is on cyclic nucleotide (cAMP and cGMP) signalling and its role in pathology. Alterations of cyclic nucleotides signals characterise several serious and widespread diseases including cardiovascular diseases. Our objective is to understand how disruption/deregulation of cyclic nucleotide signalling pathways leads to disease and if these pathways represent a potential target for therapy.
Zaccolo M. Spatial control of cAMP signalling in health and disease. Curr Opin Pharmacol. 2011 Dec;11(6):649-55