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Oxfcrispr oxford fly crispr resources

We describe a method for efficiently creating mutations in chosen Drosophila genes within a month based on the CRISPR/Cas9 system from bacteria


Double yellow and white mosaic fly - Image courtesy of N. Gompel

The following pages contain information about our protocols and reagents, new refinements of techniques and useful links to other tools, websites and discussion groups to exploit this new technology.
Our results are also described in:

Ghosh S, Tibbit C and Liu JL. Nucleic Acids Research (2016) Effective knockdown of Drosophila long non-coding RNAs by CRISPR interference. [Epub on 4 Feb 2016] [PDF] [SUPPLEMENTARY DATA]

Bassett, Kong and Liu. Journal of Genetics and Genomics (2015) A Genome-Wide CRISPR Library for High-Throughput Genetic Screening in Drosophila Cells. [PDF]
Bassett et al. Biology Open (2013) Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9 [PDF] [PubMed]
Bassett et al. Cell Reports (2013) Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system
and reviewed in:
Bassett and Liu, Journal of Genetics and Genomics (2014) CRISPR/Cas9 and genome editing in Drosophila [PDF] [PubMed]
Bassett and Liu, Methods (2014) CRISPR/Cas9 mediated genome engineering in Drosophila [PDF] [PubMed]

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