Adaptive optics is implemented in a harmonic generation microscope using a wavefront sensorless correction scheme. Both the second- and third-harmonic intensity signals are used as the optimization metric. Aberration correction is performed to compensate both system- and specimen-induced aberrations by using an efficient optimization routine based upon Zernike polynomial modes. Images of live mouse embryos show an improved signal level and resolution.
Journal article
2009-10-15T00:00:00+00:00
34
3154 - 3156
2
Air, Animals, Cytoplasm, Developmental Biology, Embryo, Mammalian, Equipment Design, Glass, Image Processing, Computer-Assisted, Mice, Microscopy, Confocal, Models, Statistical, Optics and Photonics, Refractometry, Time Factors