Here, we describe a high-throughput 3D differentiation protocol for deriving midbrain dopaminergic neurons from human pluripotent stem cells. The use of organoids has become prevalent in disease modeling, but there is a high demand for more homogeneous cultures. Our approach is advantageous for large-scale production of uniform midbrain organoids that can be maintained in diverse formats, and our reporters allow for sorting of dopaminergic neurons. The maturing long-term organoid cultures can be used as a model for the entire midbrain. For complete details on the use and execution of this protocol, please refer to Ahfeldt et al. (2020).
Journal article
2021-06-18T00:00:00+00:00
2
Cell Differentiation, Cell isolation, Neuroscience, Organoids, Stem Cells, Dopaminergic Neurons, Humans, Mesencephalon, Organoids, Pluripotent Stem Cells